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Question about simplex read quality differences between dorado duplex and dorado basecaller

Status: Open.

#1550 In nanoporetech/dorado;

· hungweichen0327 opened on Dec 28, 2025

Inconsistent Poly(A) Tail Detection in DRS Reads and Uncertainty in Poly(A) Start Site Identification

polyAIssue related to polyA tail estimationIssue related to polyA tail estimation

Status: Open.

#1545 In nanoporetech/dorado;

· PragyaKesar opened on Dec 10, 2025

Inconsistent sequence length between rna004_130bps_sup@v5.0.0@v1 and rna004_130bps_sup@v5.2.0@v1

Status: Open.

#1542 In nanoporetech/dorado;

· visanuwan opened on Dec 5, 2025

Dorado Base Calling Model Consistency: "All Context" vs. "CpG Only" for 5mC/5hmC

modsFor issues related to modified base callingFor issues related to modified base calling

questionIssue is a questionIssue is a question

Status: Open.

#1540 In nanoporetech/dorado;

· blipinskiaima opened on Nov 28, 2025

Huge difference in 6mA modification between v4.0.1_6mA@v2, v4.2.0_6mA@v3 and v5.2.0_6mA@v1

Status: Open.

#1538 In nanoporetech/dorado;

· Nirmal2310 opened on Nov 27, 2025

dorado demux crashes if <code>-o</code> argument is missed

barcodeIssues related to barcodingIssues related to barcoding

Status: Open.

Bug

#1537 In nanoporetech/dorado;

· malton-ont opened on Nov 25, 2025

Merge ubam file for dorado trimming

documentationImprovements or additions to documentationImprovements or additions to documentation

Status: Open.

#1533 In nanoporetech/dorado;

· OceaneMion opened on Nov 20, 2025

Dorado aligner gives error code -3 when merging temporary files (step needed before running dorado polish)

Status: Open.

#1513 In nanoporetech/dorado;

· mmudado opened on Oct 21, 2025

Overrepensented short sequences in the PCB114-24 data after dorado trim and pychopper

trimIssues related to adapter/primer trimmingIssues related to adapter/primer trimming

Status: Open.

#1510 In nanoporetech/dorado;

· baozg opened on Oct 3, 2025

dorado crashes when calling sup modifications 4mC_5mC 6mA on 5090

Status: Open.

Bug

#1507 In nanoporetech/dorado;

· Kirk3gaard opened on Sep 28, 2025

strand biased basecalling errors

Status: Open.

#1505 In nanoporetech/dorado;

· mrredlinger2 opened on Sep 26, 2025

Issue with SUP basecalling using RTX3080 10GB

Status: Open.

#1489 In nanoporetech/dorado;

· awjga opened on Sep 8, 2025